علوم زیستی دریا
Narges Alirahimi; Alaeddin Kordenaeej; Ayatollah Rezaei; Daryush Talei
Abstract
To evaluate the production of beta-carotene, traits and patterns of protein band in D. salina microalgae in the presence of colchicine, after microalgae cultivation, colchicine treatments randomized design concentrations of 0, 0.05 and 0.1 and 0.2% Volumetric weight was applied. Changes in growth and ...
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To evaluate the production of beta-carotene, traits and patterns of protein band in D. salina microalgae in the presence of colchicine, after microalgae cultivation, colchicine treatments randomized design concentrations of 0, 0.05 and 0.1 and 0.2% Volumetric weight was applied. Changes in growth and beta-carotene, chlorophyll A, B and total and carotenoids produced by spectrophotometer measurements, as well as electrophoresis on SDS-PAGE analysis was used to investigate the protein patterns. The results showed that concentrations of colchinicine did not result in significant differences in protein pattern in 11 samples and it just affacted the production of certain proteins.. While this treatment causes a difference in beta-carotene, chlorophyll A, B, total carotenoids and growth. Beta-carotene is the most important metabolites increased in all concentrations of colchicine. Treatment increased the carotenoids, chlorophyll A, B, and total while reduced growth. So the treatment of colchicine reduced the growth and increase the production of beta-carotene, chlorophyll A, B, total and carotenoids is in Dunaliella. While no significant effect on the pattern of protein expression. Therefore , it can be concluded that using treatment of colchicine will be useful in beta-carotene productions.